β-1,3--Glucanase production from vinasse and its application in degradation of biofilms adhered in stainless steel

Produção de β-1,3-Glucanase utilizando vinhaça e sua aplicação na degradação de biofilmes aderidos a aço inoxidável

Authors

  • Bruna Letícia Martins 1 Pró-Reitoria de Pesquisa e Pós-Graduação – PRPPG, Universidade do Sagrado Coração – Unisagrado, Bauru, SP, Brasil.
  • Luana do Amaral Bovi 1 Pró-Reitoria de Pesquisa e Pós-Graduação – PRPPG, Universidade do Sagrado Coração – Unisagrado, Bauru, SP, Brasil.
  • Cynthia Barbosa Rustiguel Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo – USP, Ribeirão Preto, SP, Brasil.
  • Fernando Tozze Alves Neves 1 Pró-Reitoria de Pesquisa e Pós-Graduação – PRPPG, Universidade do Sagrado Coração – Unisagrado, Bauru, SP, Brasil.
  • Flávio Augusto Sanches Politi Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP,Araraquara, SP, Brasil.
  • Geisiany Maria de Queiroz Departamento de Medicina, Faculdade São Leopoldo Mandic de Araras, Araras, SP, Brasil.

DOI:

https://doi.org/10.4322/2359-6643.13241

Abstract

The production of β-1,3-glucanase has been optimized using vinasse, analyzing the ideal concentration, incubation time, pH, temperature, and agitation. The biofilms were formed on stainless steel coupons for 48 hours and treated with 21.3 U/mg of enzyme for 30 minutes. The degradation efficiency of the β-1,3-glucanase produced was evaluated by the reduction of total biomass, using crystal violet staining. The enzyme showed significant biomass degradations of microbial biofilms and had the best action against Candida albicans biofilms, with total biomass reduction of 48.76%. These results are important to provide a practicable alternative for elimination of biofilms, employing agro-industrial residues that accumulate in environment

Published

2023-04-30

Issue

Section

Artigo Original